Medicago truncatula Proteomics for Systems Biology: Novel Rapid Shotgun LC-MS Approach for Relative Quantification Based on Full-Scan Selective Peptide Extraction (Selpex) - Plant Proteomics: Methods and Protocols - page 296

Table 1
Peptide target list for the Selpex approach

RT (min)

m/z (Da) TC UniRef100 unreviewed Annotation Sequence Proteotypic 120 30

1,085.49 TC112983 A2Q5A2_MEDTR RLS GGLDFTKDDENVNSQPFMR No 29 11

871.37 TC112983 A2Q5A2_MEDTR RLS GHYLNATAGTCEDMMK Yes 18 9

745.36 TC112983 A2Q5A2_MEDTR RLS WSPELAAACEVWK No 49 16

488.28 TC113878 A2Q5A3_MEDTR ATPase IGLFGGAGVGK No 12 8

801.40 TC113878 A2Q5A3_MEDTR ATPase VALVYGQMNEPPGAR No 16 9

983.01 TC171155 G7KMR3_MEDTR RSS FETLSYLPPLTEDQLAK Yes 50 16

1,047.06 TC171155 G7KMR3_MEDTR RSS KFETLSYLPPLTEDQLAK Yes 42 14

738.40 TC171155 G7KMR3_MEDTR RSS LPLFGATDSSQVLK No 32 12

718.89 TC167788 G8A0J3_MEDTR RSS FETLSYLPPLTR Yes 40 14

467.26 TC167788 G8A0J3_MEDTR RSS IIGFDNVR No 13 8

730.41 TC151243 G7J252_MEDTR RSS LPLFGATDASQVLK Yes 39 14

782.94 TC151243 G7J252_MEDTR RSS KFETLSYLPPLTR Yes 31 12

788.88 TC120394 G7K9H5_MEDTR OEE1 GASTGYDNAVALPAGGR No 12 8

618.83 TC120394 G7K9H5_MEDTR OEE1 RLTFDEIQSK No 8 7

974.98 TC135344 Similar to Q7Y1T5 (89 %) OEE3 VGGPPAPSGGLPGTLNSDEAR Yes 16 9

855.40 TC137623 G7KGT1_MEDTR Rubisco activase GLAYDISDDQQDITR No 22 10

1,152.53 TC137623 G7KGT1_MEDTR Rubisco activase MGINPIMMSAGELESGNAGEPAK No 45 15

824.91 TC137623 G7KGT1_MEDTR Rubisco activase YLEGAALGDANQDAIK No 17 9

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