Biology Reference
In-Depth Information
15
10
5
0
-5
-10
20
10
10
0
0
-10
-10
PC2
-20
-20
PC1
Fig. 2
MALDI MSI analysis of a longitudinal barley grain section, 14 days after pollination. (
a
) Multiple ion
intensity map displays distribution of fi ve distinct
m/z
values. (
b
) Histological image indicating positions of ROIs
selected for statistical analysis in CPT. (
c
) Result of PCA analysis of eight ROIs (as given in
b
) including 131
individual
m/z
values
in Google Maps. This proprietary image formats are compatible
with co-registration in the fl exImaging software.
2. The ImagePrep apparatus from Bruker Daltonics creates a fi ne
matrix aerosol in a spray chamber fi lled with nitrogen. The
aerosol is applied onto sample sections during several cycles of
deposition, incubation and drying. An optical sensor controls
the different phases of each cycle. With this technique small
droplet sizes and reproducible conditions for sample prepara-
tion are provided. Alternative ways for matrix application are
the usage of nano-spotting devices or manual pneumatic spray
devices. While with nano-spotting devices lateral resolution is
limited to at least 200
μ
m, the process of manual spraying is
