Biology Reference
In-Depth Information
Chapter 8
Making a Protein Extract from Plant Pathogenic
Fungi for Gel- and LC-Based Proteomics
Raquel González Fernández , Inmaculada Redondo ,
and Jesus V. Jorrin-Novo
Abstract
Proteomic technologies have become a successful tool to provide relevant information on fungal biology.
In the case of plant pathogenic fungi, this approach would allow a deeper knowledge of the interaction and
the biological cycle of the pathogen, as well as the identifi cation of pathogenicity and virulence factors.
These two elements open up new possibilities for crop disease diagnosis and environment-friendly crop
protection. Phytopathogenic fungi, due to its particular cellular characteristics, can be considered as a
recalcitrant biological material, which makes it diffi cult to obtain quality protein samples for proteomic
analysis. This chapter focuses on protein extraction for gel- and LC-based proteomics with specifi c proto-
cols of our current research with Botrytis cinerea .
Key words Fungal proteomics, Fungal secretome, Gel-based proteomics, LC-based proteomics
1
Introduction
Phytopathogenic fungi are one of the most damaging plant parasitic
organisms that cause serious diseases and remarkable yield losses in
crops. The biological study of these microorganisms and the interac-
tion with their hosts have experienced great advances in recent years
due to the development of modern, holistic, and high-throughput
-omic techniques, together with the increasing number of genome
sequencing projects and the development of mutants and reverse
genetics tools. Within these -omic techniques, proteomics has
become a relevant tool in plant-fungus pathosystem research
(reviewed in [ 1 - 4 ]). Molecular studies of the fungal biological cycle
and their interaction with their hosts are necessary for searching key
protein targets, and for developing new more effi cient and environ-
ment-friendly agrochemicals [ 5 , 6 ], which may open new ways for
crop disease diagnosis and protection. Thus, proteomics aims to
identify gene products with a key role in pathogenicity and virulence.
Search WWH ::




Custom Search