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Figure 1.Percentage of extremely tolerant, tolerant and sensitive Rhizobium isolates obtained from each
location. CN - Costa Nova isolates; V - Vagos isolates; SB - S. Bernardo isolates; AS - Alentejo Spring
isolates; and AWA- and AWB- Alentejo Winter isolates.
Nevertheless, when the conditions are changed and this population is submitted to a selective
pressure, the genotypes presenting tolerance ability can survive, hence yielding a tolerant
population, with lower genetic variability. However, all CN isolates showed a strong growth
inhibition at 50 mM NaCl (Table 1). This finding lead us to conclude that Rhizobium
population was not well adapted to the environmental conditions and the isolates obtained
from this location probably survive due to microsites where the soil moisture was maintained
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