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pathogens and then it became naturally suppressive to the diseases following repeated
cultivations.
Figure 1. Disease index of bacterial wilt of tomato (a), Fusarium wilt (b) and damping-off caused by
Pythium aphanidermatum (c) in unused and used pumice artificially infested with the pathogen. Date
are means ±SE of six independent experiments (a) or means ±SE of single three replicates experiment
(b,c).
Then, the physicochemical and biological properties of the unused and used pumices
were compared. After repeated cultivation, total C and N, cations, especially Ca and Mg,
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