Biology Reference
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Fig. 2
Assembling stereotaxic injector: (
A
) stereotaxic injector for bilateral injec-
tion. A double internal injector (
1
) is held by a double guide cannula (
2
) that is
mounted on a stereotaxic apparatus via a cannula holder (
3
). The internal injector
is connected to Hamilton syringe (
5
) with PE50 tubing (
4
). The Hamilton syringes
are controlled by a syringe injection pump (
6
). (a) Enlarged picture for the injector
assembling [
white box
in picture (
A
)]. 2: Single and double guide cannulas used
for hold internal injector. (
B
) Alternative assembling stereotaxic injector for uni-
lateral injection. A microsyringe pump (
7
) with a Hamilton syringe (
5
) is mounted
on stereotaxic apparatus. The microsyringe pump is controlled by a four-channel
microcontroller (
8
) (World Precision Instruments, Sarasota, FL)
Injection of Recombinant Adenovirus into Specifi c Brain Regions
An animal is anesthetized and placed on a stereotaxic apparatus. After
an incision, the tip of injector (one of the injectors for bilateral injec-
tion) is aligned with bregma and all of the coordinates are rezeroed.
(continued)
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