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Fig. 5 Infection of the rat striatum with the miRNA lentivirus induces the GRK6 knockdown. ( a ) Representative
low-magnifi cation photomicrograph of the rat intact control striatum ( left panel ) and the striatum infected with
the GRK6-GFP lentivirus ( right panel ). GFP is expressed cocistronically with GRK6 miRNA in striatal neurons as
demonstrated by GFP immunohistochemistry. ( b ) Detection of the GRK6A expression by Western blot in the
lesioned hemisphere infected with the GRK6 miRNA (GRK6) or negative control miRNA (Control) virus in com-
parison with the intact (uninfected) hemisphere. Four middle lanes are different dilutions of purifi ed recombi-
nant human GRK6A used as standards. The arrowhead points to the GRK6A band. The lower band is an
irrelevant background band. ( c ) Quantifi cation of the Western blot data for the expression of GRK6A in the
intact and lesioned hemisphere. The expression of endogenous GRK6A in the intact and lesioned (infected)
hemisphere and was compared by paired t -test: *** p < 0.001 to the intact striatum. The expression of endog-
enous GRK6A in the lesioned (infected) hemispheres of rats infected with the control (Control miRNA) and
GRK6 miRNA viruses was compared by unpaired t -test ( p < 0.05). ( d ) Detection of the GRK6B expression by
Western blot in the lesioned hemisphere infected with the GRK6 miRNA (GRK6) or negative control miRNA
(Control) virus in comparison with the intact (uninfected) hemisphere. Three middle lanes are different dilutions
of recombinant GRK6B used as standards (the lysate of HEK293 cells transfected with rat GRK6B). ( e ) The
expression of endogenous GRK6B in the intact and lesioned (infected) hemisphere was compared by paired
t -test: * p < 0.05; ** p <0.01 to the intact striatum. The expression of endogenous GRK6B in the lesioned
(infected) hemispheres of rats infected with the negative control (Control miRNA) and GRK6 miRNA viruses was
compared by unpaired t -test ( p < 0.001). (Reproduced with some modifi cations with permission from [ 68 ] )
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