Application of Viral Vectors to Motor Neuron Disorders - Viral Vector Approaches in Neurobiology and Brain Diseases - page 226

Fig. 2 Positioning of mouse pups for AAV6 vector injection either in individual
muscles or in the lateral brain ventricle ( lower right panel )

After all injections have been performed, the anesthesia is reversed
by subcutaneous injection of 5 l of atipamezole (1 mg/ml). The

pup is maintained on a heating mattress until it is fully awake and
can be brought back to its cage.

μ

1. Material preparation . Injections are performed by mean of
30G insulin syringes. Fill the syringe with ultrapure sterile
water and slowly empty it while making sure no air bubble
remains. Prepare the virus suspension by adding 0.1 % Fast

3.3.2 Intracere-
broventricular Injection