Biology Reference
In-Depth Information
Glutamine (200 mM, 400×) (Gibco)
Penicillin (10,000 U/ml)/streptomycin (10,000
μ
g/ml) (100×)
mixture (Invitrogen)
Astrocyte culture medium: 10 % FBS (Gibco), 0.5 mM glutamine,
0.1 % penicillin/streptomycin in DMEM (Gibco)
Needles: 0.9 × 50 mm (20 gauge), 0.8 × 40 mm, and 0.8 × 300 mm
(21 gauge)
4 % Paraformaldehyde (w/v) (Sigma), highly toxic
Normal goat serum (NGS) (Gibco)
Triton X-100 (Sigma)
Primary antibody 2B4 (gift from Yvon Trottier, CNRS/INSERM,
Illkirch, France) [ 48 ]
Secondary antibody: coupled to fl uorophores (Jackson
Immunoresearch Laboratory) or biotin (Vector Laboratories)
Vectastain Elite ABC detection kit (Vector Laboratories)
2.4.2 Immunostaining
for Mutant Htt
Animal health drugs:
2.5 LV Rodent
Model for HD
Domitor (Covéto)
Ketamine (Covéto)
Xylazine (Covéto)
Isofl urane (CSP)
Scalpels
Stereotaxic frame
Electric razor
Binocular microscope
For the cannulas:
2.5.1 Stereotaxic
Injections
34-Gauge blunt-tip (Phymep)
Hamilton syringe (Hamilton)
Polyethylene catheter (Phymep)
NeuN antibody (Millipore, MAB377)
S100
2.5.2 Immunostaining
for Tropism
antibody (Sigma, S2532)
Gelatin from pig skin, type A (Sigma)
Alexa Fluor (Invitrogen), store in the dark
Basic MOM (mouse on mouse) kit (Jackson Immunoresearch
Laboratory)
Fluorsave reagent (Invitrogen)
β
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