Using Viral Vectors to Study the Memory Trace in Mice - Viral Vector Approaches in Neurobiology and Brain Diseases - page 133

2.1.2 Materials

Scale for weighing mouse
Scalpel
Dissection scissors
Kimwipes
Cotton swabs (Q-tips)
Eye lubrication drops
Antibiotic gel
Small animal stereotactic frame (ASI Instruments, Inc.)
Syringe pump (Cat# SP100i, World Precision Instruments, Inc.)
High speed micro drill [Cat# 18000-17, Fine Science Tools (FST),
Inc.]
0.5 mm diameter carbon steel burr (drill bit) (Cat# 19007-05,
FST, Inc.)
Heating pad
EZ clip applier (Cat# 59023, Stoelting Co.)
EZ clips (Cat# 59027, Stoelting Co.)
Fine-tip markers (washable, Sharpie)
Hamilton syringe (1801N, 10.0 μ l, Cat# 84875, Hamilton

Company)
Injection pipette with tubing
Patch clamp glass pipette (1.65/1.1 mm OD/ID) (Cat#
PG10165-4, World Precision Instruments, Inc.) with the
tip pulled to a length greater than the injection depth and
the other end glued to PE20 tubing (25-30 cm).

The coordinates for the dorsal part of the LA may vary. We use the
following coordinates (from Bregma) for mice ( F1 of C57Bl/6 ×
129 SvEv genetic background) weighing between 20 and 25 g:

2.1.3 Injection
Coordinates for Lateral
Amygdala (from Bregma)
in Mice

±3.5 mm mediolateral
−1.4 mm posterior
5.0 mm dorsoventral

2.2 Fear
Conditioning (Training
and Testing)

NIR Fear Conditioning System for Mouse (Cat# MED-VFC-
NIR-M, Med Associates, Inc.)
Video Fear Conditioning Interface Package (Cat# MED-SYST-
VFC, Med Associates, Inc.)

2.3 catFISH Assay

Cryostat (e.g., Leica 1850-3-1)
Embedding medium (e.g., OCT compound, Cat# 4583, Sakura
FineTek, Inc.)
Brain matrix

2.3.1 Tissue Sectioning