Biology Reference
In-Depth Information
solution containing in mM: 117.5 CsMeSO 4, 15.5, CsCl, 10
TEACl, 8 NaCl, 1.25 NaH 2 PO 4 , 10 HEPES, 0.25 EGTA, 4
MgATP, and 0.3 NaGTP, pH = 7.3, osmolarity ~290 mOsm.
Giga seals should be obtained before break in. During record-
ing, series resistance and input resistance should be monitored
to ensure stability of cell access and health.
(e) Synaptic stimulation is provided by an extracellular electrode
fi lled with ACSF and placed over the axons of the afferents
that contact the patched neurons. Data are collected through
the microelectrode amplifi er and data acquisition/analysis
system.
4
Typical/Anticipated Results
Figure 3 shows a successful injection of a GFP-expressing Sindbis
virus in the CA1 layer and the dentate gyrus of a rat hippocam-
pus after 24 h of expression. Note that the virus principally infects
principal excitatory neurons in these areas, displaying high
Fig. 3 Successful expression of GFP-expressing sindbis virus in the CA1 pyrami-
dal neurons ( top ) or dentate gyrus granule cells ( bottom ) in 21-day-old Sprague-
Dawley rat 24 h after in vivo infection
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