Essential Methods of Plant Sample Preparation for Light Microscopy - Plant Cell Morphogenesis: Methods and Protocols - page 22

16. Alternatively, place samples into a small aluminum foil mold
fi lled with cryoembedding medium. To prepare the mold, fold
a rectangular sheet of thicker Al foil around a small cover slips
box. It is easier to handle small specimens and arrange them
into desirable position using forceps or needle. Freeze the
mold on cryobar inside the cryostat chamber or immerse the
mold into isopentane supercooled with liquid nitrogen. Peel
away the Al foil and fi x frozen block on the chuck with drop of
cryoembedding medium.

Acknowledgment

This work has been supported by the COST-LD11017 project.

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