Laser Microdissection of Plant Cells - Plant Cell Morphogenesis: Methods and Protocols - page 242

Table 1
Overview of LMD application in plants

Downstream
analysis Species Tissue LMD system Fixation Embedding RNA extraction Amplifi cation References

Arabidopsis
thaliana

Embryo PixCell II

(Arcturus)

None OCT Absolutely RNA

Nanoprep Kit
(Stratagene)

MessageAmp
Kit (Ambion)

Microarray [ 25 ]

Brassica napus Seed PALM

MicroBeam
(Zeiss)

None OCT Total RNA

(columns)/
mRNA (beads)

According to
ref. [ 26 ]

Lipids, starch,
metabolites,
transcriptomics

[ 23 ]

Citrus
clementina

Leaf AS LMD (Leica) None OCT RNeasy Micro

Kit (Qiagen)

TargetAmp Kit
(Epicentre
Biotech.)

Microarray [ 16 ]

Hordeum
vulgare

Grain PALM

MicroBeam

None None NA NA Proteomic [ 12 ]

Medicago
truncatula

Root cortex PALM

MicroBeam

None TFM RNeasy Plant

Extraction Kit
(Qiagen)

WT-Ovation
Kit
(NuGEN)

Microarray [ 27 ]

Oryza sativa Shoot apex Veritas LMD

LCC1704
(Arcturus)

3:1 EtOH: AA Paraffi n—

microwave

PicoPure RNA
isolation kit
(Arcturus)

Quick Amp
Labelling Kit
(Agilent)

Microarray [ 22 ]

Crown roots Veritas LMD

LCC1704

99.5 % EtOH
or acetone

Paraffi n—
microwave

PicoPure RNA
isolation kit

Quick Amp
Labelling Kit

Microarray [ 13 ]

Anthers Veritas LMD

LCC1704

3:1 EtOH: AA Paraffi n—

microwave

PicoPure RNA
isolation kit

None Microarray [ 15 ]

Aleurone,
endosperm

AS LMD Acetone, 3:1

EtOH: AA

CMC,
paraffi n

PicoPure RNA
isolation kit

Based on ref.
[ 7 , 8 ]

qRT-PCR [ 17 ]