Biology Reference
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Fig. 2
Cardboard origami for paraffi n embedding (
a
)—bend along the dashed
lines to get a boat suitable for paraffi n embedding. Grey indicates upper sided of
the cardboard. (
b
) Arrange the objects into paraffi n block so that they might be
easily separated after embedding. (
c
) To adjust correct blade clearance angle,
the shape of the blade should be respected. Incorrect angle of the blade can
either crush (
II
) or scrape (
III
) the block instead of cutting (
I
). (
e
) Sectioning of
paraffi n-embedded object on the microtome and ribbon formation, (
d
) subse-
quent fl attening of sections on slides
18. Trim paraffi n blocks into desirable size and shape for easy
sectioning. Proper trimming allows easier achievement of
ribbons during sectioning (Fig.
2b
).
19. Fix the chuck into the microtome clamp. Use microtome
clamp to adjust object into optimal position according to cut-
ting plane. Reassure that the clamping mechanism is tightened
securely before trimming and sectioning.
20. Adjust proper microtome knife angle (
see
Note 14
; Fig.
2c
).
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