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2. In the PEG medium, only non-metabolized osmotic solute is
present and pollen utilizes just internal energy sources. In the
case of sucrose medium, sucrose serves as an energy source as
well as osmotic agent and abnormal thick callosic depositions
are formed in the cell wall of pollen tubes. Many parameters of
growth and pollen tube physiology thus differ according to
the cultivation medium used.
3. For the short culture times required, the media do not need to
be sterilized.
4. Polypropylene test tubes are not recommended as cytofectin
binds to polypropylene.
5. Due to the ODN effect on de novo transcription/translation
(and possibly also mRNA stability), delayed manifestation of
the lack of target protein should be expected.
Acknowledgment
Pollen tube research in the Žárský lab is supported by the Czech
Grant Agency grant GACR 13-19073S to M. P.
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