Mechanical Measurements on Living Plant Cells by Micro- indentation with Cellular Force Microscopy - Plant Cell Morphogenesis: Methods and Protocols - page 141

4. Check the timescale of sample growth compared to the time
needed for the measurements. The CFM setup can be adapted
to perform measurements horizontally instead of vertically. In
case the organ presents a fast gravitropic response when laid
horizontally, it can be then kept in an upright position during
the experiments with a setup modifi ed to measure
horizontally.

Acknowledgments

We gratefully acknowledge all those involved in the development
of the CFM, Dimitris Felikis, Simon Muntwyler, Felix Beyeler,
Brad Nelson, Cris Kuhlemeier, and Michal Hufl ejt, as well as those
who helped to perfect the protocol in the fi rst experiments, Petra
Kochova and Alain Weber. We thank Gabriella Mosca, Pierre
Barbier de Reuille, and Sarah Robinson for discussions and Willi
Tanner for custom machine work. This work was supported by
SystemX.ch and the University of Bern.

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ć K, Roduit C, Simonovi ć