Multicompartment Lipid Nanocarriers for Targeting of Cells Expressing Brain Receptors - Self-Assembled Supramolecular Architectures: Lyotropic Liquid Crystals - page 345

SH-SY5Y cells by retinoic acid and the expression of the BDNF receptor
(TrkB). After the induction of a reduced cellular viability by the treatment
with retinoic acid, the phenomenon of neuroprotection by BDNF and the
effect of potentiation of this neurotrophic factor by EPA and liquid crystalline
lipid nanocarrier formulations (containing MO/DOPE-PEG 2000 /EPA or
DMPC/DOPE - PEG 2000 /EPA) were evidenced. The human neuroblastoma SH-
SY5Y cell line, treated with retinoic acid, was proven as a good model to study
the effi ciency and the toxicity of neuroprotective agents associated with func-
tionalized lipid NP formulations. The polyunsaturated fatty acid EPA showed
a real effect of potentiation of the neurotrophic factor BDNF alone and in a
combination with liquid crystalline monoolein-based nanoassembles.
ACKNOWLEDGMENT
Dr. Jean-Marc Muller (CNRS UMR 6187) is gratefully acknowledged for
providing the neuroblastoma cell line SH-SY5Y. The experimental facilities at
the synchrotron Diamond Light Source (UK) and the Cell-Imaging platform
of IFR 141 (IPSIT), “Institute of Therapeutic Innovation: From Fundamental
to Medicines,” are acknowledged. This contribution is dedicated to the memory
of Dr. Michel Ollivon.
REFERENCES
Alfons , K. , and Engstrom , G. ( 1998 ). Drug compatibility with the sponge phases formed
in monoolein, water, and propylene glycol or poly(ethylene glycol). Journal of
Pharmaceutical Sciences , 87 , 1527 - 1530 .
Almeida , A. J. , and Sauto , E. ( 2007 ). Solid lipid nanoparticles as a drug delivery system
for peptides and proteins. Advanced Drug Delivery Reviews , 59 , 478 - 490 .
Anderson , D. M. , and Wennerstr ö m H. ( 1990 ). Self diffusion in bicontinuous cubic
phases, L3 phases and microemulsions. Journal of Physical Chemistry , 94 , 8683 -
8694 .
Angelov , B. , Angelova , A. , Papahadjopoulos - Sternberg , B. , Lesieur , S. , Sadoc , J. - F. ,
Ollivon , M. , and Couvreur , P. ( 2006 ). Detailed structure of diamond - type lipid cubic
nanoparticles . Journal of the American Chemical Society , 128 , 5813 - 5817 .
Angelov , B. , Angelova , A. , Garamus , V. M. , Le Bas , G. , Lesieur , S. , Ollivon , M. , Funari ,
S. S. , Willumeit , R. , and Couvreur , P. ( 2007 ). Small - angle neutron and X - ray scatter-
ing from amphiphilic stimuli-responsive diamond type bicontinuous cubic phase.
Journal of the American Chemical Society , 129 , 13474 - 13479 .
Angelov , B. , Angelova , A. , Vainio , U. , Garamus , V. M. , Lesieur , S. , Willumeit , R. , and
Couvreur P. ( 2009). Long living intermediates during a lamellar to a diamond-cubic
lipid phase transition: A SAXS investigation. Langmuir , 25 , 3734 - 3742 .
Angelov , B. , Angelova , A. , Mutafchieva , R. , Lesieur , S. , Vainio , U. , Garamus , V. M. ,
Jensen , G. V. , and Pedersen , J. S. ( 2011b ). SAXS investigation of a cubic to a sponge