Multicompartment Lipid Nanocarriers for Targeting of Cells Expressing Brain Receptors - Self-Assembled Supramolecular Architectures: Lyotropic Liquid Crystals - page 340

(a) (b)

(c)

Figure 11.11 Confocal fl uorescence microscopy images (oil objective 63×/1.4) of SH-
SY5Y cells incubated with retinoic acid (10 μM) at 37°C under 5% CO 2 for 4 days and
then fi xed on glass slides: (a) Fluorescence image (zoom × 2), (b) Nomarski image, and
(c) the superposition of the images (a) and (b). Image size: 73.25 × 73.25 μ m 2 . The scale
bar corresponds to 10 μ m.

11.12 shows the results for the relative viability of the cells treated with the
peptide BDNF alone or with decreasing concentrations of the MO/DOPE-
PEG 2000 /EPA (83/2/15 mol %) nanodispersion in the presence or absence of
BDNF. The control experiments corresponded to differentiated cells, which
were not subjected to neuroprotective treatment. The viability of cells treated
with BDNF alone was 108 0) (Fig. 11.12). It evidenced that the

neurotrophic factor BDNF essentially increases the survival of differentiated
neuroblastoma cells, which exhibited initial cellular viability around 40%.
Thus, BDNF was proven to act as a neuroprotectant that maintains cellular
survival and promotes the recovery of the SH-SY5Y cells.
A maximal cellular viability of 123% was found for treatment with BDNF
added to MO/DOPE-PEG 2000 /EPA (83/2/15 mol %) nanocarrier formulations
with a total lipid concentration of 4.21

± 6% ( C lipid =

1 0 − 6 nmol lipid/cell).
In the absence of BDNF, the cellular viability decreased to 90% for the same
formulation at equal concentration of the neurotrophin potentiating agent

1 0 − 7 M (i.e. 2 × ×