Biology Reference
In-Depth Information
Table 14.1: Selected Examples of Genetic Modifications of Arthropods Using Multiple Methods.
Project goals
Selected reference(s)
Gal4-US targeted gene expression
Gene expression in
Aedes aegypti
evaluated at a specific insertion site
Kokoza and Raikhal 2011
Gene knockout using Transcription Activator-Like Effector Nucleases (TALENs)
Modiication of
Gryllus bimaculatus
genome
Watanabe et al. 2012
Gut microbe affects malaria transmission
Naturally occurring gut microbe reduces infection of
Anopheles gambiae
by
Plasmodium
Cirimotich et al. 2011
Homing endonucleases for precise modification of the genome
Transformation of
Ae. aegypti
Traver et al. 2009
Transformation of
An. gambiae
Windbichler et al. 2011
Medea
as a driver for population replacement
Medea
tested for population replacement in
Drosophila
Chen et al. 2007
Medea
is a
m
aternal-
e
ffect
d
ominant
e
mbryonic
a
rrest construct
that kills all progeny not inheriting the
Medea
- from the maternal
or paternal genome. Such elements will spread if released Into a
population above a threshold frequency.
Paratransgenesis
Transgenic symbiont from
Rhodnius
, the vector of Chagas disease
Durvasula et al. 2008
Viral paratransgenesis in
An. gambiae
Ren et al. 2008
Paratransgenesis of sandfly by gut symbiont (
Bacillus subtilis
containing
a green fluorescent protein gene)
Hurwitz et al. 2011b
Produce novel silk or proteins
Chimeric spider-silk moth silk produced in
Bombyx mori
Teule et al. 2012
Production of therapeutic proteins in
B. mori
Royer et al. 2005, Tatematsu et al.
2010, Zhao et al. 2010
RIDL for control of pest insects
Lethality driven by the dominant tetracycline-controlled trans-activator
that results in lethality of progeny of transgenic
D. melanogaster
Horn and Wimmer 2003
Late-acting dominant lethal system for mosquito control
Phuc et al. 2007
Ae. aegypti
modified by RIDL also contain genes that make females
flightless
Fu et al. 2010
Conditional embryonic lethality in Medfly,
Ceratitis capitata
Schetelig et al. 2009a
Conditional embryonic lethality in Caribbean fruit fly,
Anastrepha suspensa
Schetelig and Handler 2012a
RNAi for gene silencing or pest control
Control of western corn rootworm in corn
Baum et al. 2007
Control of aphids
Jaubert-Posamai et al. 2007,
Borgio 2010, Pitino et al. 2011
(
Continued
)
