Biology Reference
In-Depth Information
taxonomic markers for these characteristics add to what can be measured
simply and directly?” and noted “There are very few instances where con-
trol programmes are guided by data on sibling species compositions of vector
populations.”
Likewise,
Curtis (2002)
noted that “Much emphasis is placed on studies of bio-
chemical or molecular resistance mechanisms, but it was painstaking and direct
field testing, rather than these molecular or biochemical studies, that revealed
“
…
resistance to pyrethroid insecticides in malaria vectors in South Africa.
Curtis
(2002)
concluded, “that molecular methods in medical entomology should not
be an end in themselves. They should be adopted only after careful investiga-
tion shows that they can pass the “so what?” test, that is,
could they solve real
problems that are not already solvable by simpler and cheaper methods
.”
There are genuine benefits to molecular methods, although they are not
panaceas. Because the field is yet young, it is difficult to know
a priori
whether
a particular molecular tool will aid in solving a specific problem. However, it is
appropriate to ask whether there are more-rapid and less-expensive methods
that can resolve the question.
Relevant Journals
Biochemical Systematics and Ecology
, Elsevier Press
Conservation Genetics
, Springer Publishers
Molecular Ecology
, Blackwell Publ.
Molecular Ecology Notes
, now
Molecular Ecology Resources
, Blackwell Publ.
References Cited
Abate, T., (1993). Is the invading medfly eligible for California residency.
Bioscience
43
: 3-6.
Agusti, N., De Vicente, M.C., and Gabarra, R., (1999). Development of sequence characterized
region (SCAR) markers of
Helicoverpa armigera
: a new polymerase chain reaction-based
technique for predator gut analysis.
Mol. Ecol.
8
: 1474-1476.
Alamalakala, L., Skoda, S.R., and Foster, J.E., (2009). Amplified fragment length polymorphism
used for inter- and intraspecific differentiation of screwworms (Diptera: Calliphoridae).
Bull.
Entomol. Res.
99
: 139-149.
Aquadro, C.F., Noon, W.A., and Begun, D.J., (1992). RFLP analysis using heterologous probes. In:
Hoelzel, A.R., Ed.,
Molecular Genetic Analysis of Populations. A Practical Approach
, IRL Press,
Oxford, UK, pp. 115-158.
Archak, S., Meduri, E., Kumar, P.S., and Nagaraju, J., (2007). InSatDb: a microsatellite database of
fully sequenced insect genomes.
Nucleic Acids Res.
35
: D36-D39.
Arias, R.S., Blanco, C.A., Portilla, M., Snodgrass, G.L., and Scheffler, B.E., (2011). First microsatellites
from
Spodoptera frugiperda
(Lepidoptera: Noctuidae) and their potential use for population
genetics.
Ann. Entomol. Soc. Am.
104
: 576-587.
Arimura, G., Tashiro, K., Kuhara, S., Nishioka, T., Ozawa, R., and Takabayashi, J., (2000a). Gene
responses in bean leaves induced by herbivory and by herbivore-induced volatiles.
Biochem.
Biophys. Res. Commun.
277
: 305-310.
