Biology Reference
In-Depth Information
CHAPTER 13
Insect Population Ecology and
Molecular Genetics
Chapter Outline
13.1 Overview 592
13.2 Introduction 593
13.3 What is Molecular Ecology? 593
13.4 Collecting Arthropods in the Field for Analysis
594
13.5 Molecular Ecological Methods
595
13.5.1 Allele-Specific PCR 596
13.5.2 Allozymes (Protein Electrophoresis) 596
13.5.3 Amplified Fragment Length Polymorphisms (AFLP-PCR)
599
13.5.4 Double-Strand Conformation Polymorphism (DSCP)
599
13.5.5 Heteroduplex Analysis (HDA)
600
13.5.6 Microarrays 601
13.5.7 Microsatellites 602
13.5.8 RFLP Analysis 604
13.5.9 PCR-RFLP 604
13.5.10 RAPD-PCR 605
13.5.11 Sequencing 606
13.5.11.1 DNA Barcoding 607
13.5.12 Single Nucleotide Polymorphism (SNP) Markers
608
13.6 Analysis of Molecular Data
608
13.6.1 Allozymes 609
13.6.2 Microsatellites 612
13.6.3 RAPD-PCR 613
13.6.4 RFLPs 613
13.6.5 Sequencing 614
13.7 Case Studies in Molecular Ecology and Population Biology
614
13.7.1
Genetic Variability in the Fall Armyworm: Incipient Species or Multiple Species?
614
13.7.2
Analyses of Natural Enemies
617
13.7.2.1
Analyses of Adult Parasitoid Wasps to Reveal Larval Hosts (MAPL)
617
13.7.2.2
Analyses of the Establishment of a Parasitoid Released in a Classical Biological Control
Program 618
13.7.2.3
Gut Analyses of Predatory Insects to Reveal Prey Species
618
13.7.3
Population Isolation and Introgression in Periodical Cicadas
620
 
 
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