Biology Reference
In-Depth Information
Figure 9.2
Steps in transforming
D. melanogaster
with
P
-element vectors. See text for details.
(A) Construct or choose an appropriate
P
vector containing the exogenous DNA
and appropriate regulatory elements, as well as a marker gene(s) of interest. In
this example, the marker gene is a wild-type version (
white
+
or
w
+
) of the mutant
gene for
white
(
w/w
) eyes. The wild-type allele (producing red eyes) is dominant
over
white
, so if a single copy of
w
+
is present, the fly will have red eyes. This
vector is unable to insert into the chromosome because it can't produce its own
transposase. The transposase gene has been eliminated in this vector.
Select a
helper plasmid
, such as p
π
25.7wc, that contains a complete DNA
sequence coding for transposase. This vector is unable to insert into chromosomes
