Biology Reference
In-Depth Information
Figure 5.3
Genetic maps of the multiple cloning sites of some pUC plasmids. The multiple cloning site is inserted in the
lacZ
gene but
does not disrupt
lacZ
gene function. However, if exogenous DNA is cloned into this site, then
lacZ
gene function is disrupted, which indi-
cates that insertion was successful. This allows the genetic engineer to detect colonies of
E. coli
containing recombinant plasmids because
these colonies are white rather than blue if the
E. coli
are grown on a substrate with 5-bromo-4-chloro-3-indolyl-
β
-
d
-galactoside (X-Gal).
The pUC plasmids also contain an ampicillin resistance gene.
