Biomedical Engineering Reference
In-Depth Information
innocua
was obtained at 450 MPa and 5 mg/l nisin. For this treatment, the two
microorganisms were not detectable after 1 month of storage at 4 °C. However,
nisin showed no effect in preventing growth of
E. coli
in samples stored at 20 °C
after pressurization. Nevertheless, counts of
L. innocua
were about 5 log cycles
lower than controls after 5 days of storage at 20 °C. This could be explained by the
greater reduction of viable counts obtained for this bacterium in the combined treat-
ment, but also to a post-process protective effect of the added nisin.
Another approach for application of nisin in egg was immobilization of the bac-
teriocin. Liquid egg white inoculated with
L. monocytogenes
Scott A was stored in
glass jars that were coated with a mixture of polylactic acid (PLA) polymer and
nisin, and stored at 4 and 10 °C (Jin
2010
).
Listeria
cells in control and PLA coating
without nisin samples declined 1 log CFU/ml during the fi rst 6 days at 10 °C and
during 28 days at 4 °C, and then increased to 8 or 5.5 log CFU/ml. In comparison,
the treatment of PLA coating with 250 mg nisin rapidly reduced the cell numbers of
Listeria
in liquid egg white to undetectable levels after 1 day, and the bacterium
remained undetectable throughout the whole storage periods (48 days at 10 °C and
70 days at 4 °C). Another study reported that a PLA coating containing allyl isothio-
cyanate in combination with 250 mg nisin reduced the population of a three-strain
Salmonella enterica
cocktail inoculated in liquid egg white to an undetectable level
after 21 days of storage (Jin et al.
2013
) .
References
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