Biology Reference
In-Depth Information
Fig. 1. Vectors for transposon mutagenesis of
Borrelia burgdorferi
. Both pMarGent
(A)
and pGKT
(B)
contain the high-copy origin of replication (ColE1) in
Escherichia
coli
but are suicide vectors in
B. burgdorferi
. Therefore, gentamicin-resistance (encoded
by
aacC1
)in
B. burgdorferi
can only be conferred by transposition of the transposon
(region shaded gray). In
E. coli
, double selection of the antibiotics kanamycin and
gentamicin can be imposed for pGKT, making it more stable than the progenitor vector
pMarGent, which is only gentamicin resistant. Note that for pGKT transformation
of
B. burgdorferi
, kanamycin resistance is lost once transposition has occurred. ITR,
inverted terminal repeat;
kan
, kanamycin-resistance cassette;
himar1
, gene encoding
transposase.
Search WWH ::
Custom Search