Biology Reference
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d
a
c
b
A
B
f
e
C
D
Fig. 1. Surgical processing of the cotton rat nose for recovery of colonizing
Staphy-
lococcus aureus
.
(A)
With the sacrificed animal lying on its back, secure the chin
between the thumb and forefinger and slide the scalpel blade down the prominent front
teeth (
a
) until it contacts the soft tissue of the lips (
b
).
(B)
Slide the scalpel blade under
the nostrils (
c
) and back along the bone of the bridge of the nose (
d
) removing the nose
along with the cartilage and skin from the bridge of the nose.
(C)
Grasp the skin and
cartilage of the bridge of the nose with forceps and laterally bisect the nostrils (
e
) with
scissors.
(D)
The bisected cotton rat nose ready to be vortexed. The arrow (
f
) indicates
the lumen of the nostril bisected by the cut. The dashed line indicates the midline of
the nose where the cut was made.
12. Repeat the procedure with each animal and each group until completed.
13. Discard the sacrificed animals as appropriate for the facility (
see
Note 24
).
14. Just prior to inoculating agar plates, vortex samples again on maximum for 10 s.
15. Inoculate an individual TSA + 7.5% NaCl (
see
Note 25
) bacterial agar plate
with 100 μL (
see
Note 19
) of supernatant from each sample and spread the
solution evenly on the agar surface with a disposable spreader.
16. Repeat
step 15
for each sample.
17. Incubate TSA + 7.5% NaCl plates for 48 h and then count the colonies (
see
Note 26
).
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