Biology Reference
In-Depth Information
bioluminescence and the degree of kanamycin resistance in the bacterium is
dependent upon the endogenous promoter directly upstream of the site at which
the
lux
transposon integrates.
3. The following conversion factors are useful in adjusting cell suspensions to the
desired levels:
S. aureus
Xen 29 OD
600
1.0 = 2.8 × 10
8
CFUs/mL,
P. aeruginosa
Xen5OD
600
1.0 = 7.5 × 10
8
, and
P. mirabilis
Xen 44 OD
600
1.0 = 5.4 × 10
8
CFUs/mL.
4. Incubation time for
S. aureus
Xen 29 to reach 10
3
CFUs/Catheter = 30 min,
10
4
CFUs/Catheter=1h,and10
5
CFUs/Catheter=2hin10
5
CFUs/mL of
suspension. For
P. aeruginosa
Xen 5 to reach 10
3
CFUs/Catheter = 10 min,
10
4
CFUs/Catheter = 1.5 h, and 10
5
CFUs/Catheter=3hin10
5
CFUs/mL
of suspension. The above values are valid for 14-gauge, 1-cm segments of
Teflon intravenous catheter. The concentration of cell suspension and time
required to reach desired inoculum varies with surface area and the type of
material.
5. The challenge doses required to establish chronic UTI with
P. aeruginosa
Xen 5
is 1.6 × 10
6
CFUs/catheter or 2.0 × 10
6
CFUs/50-μL of suspension and with
P.
mirabilis
Xen 44, 7.1 × 10
3
CFUs/catheter or 1.3 × 10
4
CFUs/50-μL of suspension.
References
1. Contag, C. H., Contag, P. R., Mullins, J. I., Spilman, S. D., Stevenson, D. K., and
Benaron, D. A.. (1995) Photonic detection of bacterial pathogens in living hosts.
Mol. Microbiol
.
18
, 593-603.
2. Burns-Guydish, S. M., Olomu, I. N., Zhao, H., Wong, R. J., Stevenson, D. K.,
and Contag, C. H. (2005) Monitoring age-related susceptibility of young mice to
oral
Salmonella enterica
serovar Typhimurium infection using an
in vivo
murine
model.
Pediatr. Res
.
58
, 153-158.
3. Francis, K. P., Yu, J., Bellinger-Kawahara, C., Joh, D., Hawkinson, M. J., Xiao, G.,
Purchio, T. F., Caparon, M. G., Lipsitch, M., and Contag, P. R. (2001) Visualizing
pneumococcal infections in the lungs of live mice using bioluminescent
Strepto-
coccus pneumoniae
transformed with a novel gram-positive lux transposon.
Infect.
Immun
.
69
, 3350-3358.
4. Rocchetta, H. L., Boylan, C. J., Foley, J. W., Iversen, P. W., LeTourneau, D.L.,
McMillian, C. L., Contag, P. R., Jenkins, D. E., and Parr, T. R., Jr. (2001)
Validation of a noninvasive, real-time imaging technology using bioluminescent
Escherichia coli
in the neutropenic mouse thigh model of infection.
Antimicrob.
Agents Chemother
.
45
, 129-137.
5. Francis, K. P., Joh, D., Bellinger-Kawahara, C., Hawkinson, M. J., Purchio, T. F.,
and Contag, P. R. (2000) Monitoring bioluminescent
Staphylococcus aureus
infec-
tions in living mice using a novel
luxABCDE
construct.
Infect. Immun
.
68
,
3594-3600.
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