Bioluminescent Imaging of Bacterial Biofilm Infections In Vivo - Bacterial Pathogenesis: Methods and Protocols - page 217

(a)

(b)

Fig. 1. (A) In vivo bioluminescence monitoring of S. aureus Xen29 in the mouse
model of biofilm infection during treatment with rifampin. Effect of 4 days of rifampin
treatment on a 23-day-old biofilm. Total number of photons detected per second over
the infected catheter was determined using an IVIS ® Imaging System and LivingImage ®
software, plotted with respect to time. Each mouse was implanted with two catheters
and each data point is the mean SD of 2-3 mice. Arrow indicates the day of antibiotic

administration. Data are averages of results from three to four separate experiments.
(B) Correlation between bioluminescence and traditional colony-forming units (CFUs)
for 4-day rifampin treatment on a 23-day old biofilm. Viable counts are reported as
CFUs per catheter, and bioluminescence is represented as Photons/Sec/Catheter using
IVIS ® Imaging System. Each data point is the mean

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SE of three to four catheters.
Mice were first imaged for bioluminescence followed by removing and extracting the
bacteria from catheters for CFU determinations. Reproduced with permission from
ASM Publication Department.

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