Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages - Bacterial Pathogenesis: Methods and Protocols - page 130

Table 1
Commercially Available Antibodies and Fluorescent Probes that Label Intracellular Organelles of Murine Macrophages

Compartment Marker/probe Antibodies Comments/reference

Early endosomes EEA-1 Goat -EEA-1 (N-19) Rab5 effector; Santa Cruz

Biotechnologies (use at 1:50)

J. T. August, DHSB a (use at 1:400)

Late endosomes LAMP-1 Rat monoclonal -LAMP-1 (1D4B)

J. T. August, DHSB a (use at 1:400)

Lysosomes LAMP-1 Rat monoclonal -LAMP-1 (1D4B)

Cathepsin D Rabbit -cathepsin D Dako Corporation (use at 1:200)

Endoplasmic reticulum Calnexin Rabbit -calnexin-CT Transmembrane protein; Stressgen

Bioreagents (SPA-860, use at
1:1000)

Calreticulin Rabbit -calreticulin Membrane-associated; Affinity

Bioreagents (use at 1:1000)

PDI Mouse -PDI Lumenal; Stressgen Bioreagents

(SPA-860, use at 1:100)

ERGIC COPI Rabbit --COP Affinity Bioreagents (use at 1:200,

requires SDS extraction), also labels
the Golgi apparatus

Golgi apparatus GM130 Mouse -GM130 BD Transduction Laboratories (use

at 1:100)

Autophagosomes Monodansylcadaverine Autofluorescent, accumulates in

autophagosomes

a Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by the University of Iowa, Department
of Biological Sciences, Iowa City, IA.