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the existing air bubble at the top and center of the viewing glass (level). Begin
dispensing the remaining wash A into the chip while holding the bubble steady
at the top center. Dispense until a small amount of wash A is visible in the empty
tip (opposite septum), and remove the pipette. A clear glass without air should
remain.
Acknowledgments
This work was supported by the NIH-NRRI grant P20RR020185 (J.M.V.)
and the Intramural Research Program of the National Institute of Allergy and
Infectious Diseases, National Institutes of Health (F.R.D.).
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