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9. Sequence data using these primers will contain a small amount of vector DNA
(including the inverted terminal repeat sequence that serves as a recognition
site for the transposase: 5ยด-ACAGGTTGGCTGATAAGTCCCCGGTCT). Also,
because of the large number of highly related gene families in the
B. burgdorferi
genome, some BLAST results will return multiple genes, requiring more detailed
analysis to identify the exact location of the transposon insertion.
Acknowledgments
We gratefully acknowledge the technical expertise of Virginia Tobiason
for construction of pGKT, and Jon Krum for design of gentamicin-specific
oligonucleotides. We thank Gary Hettrick for graphical expertise and Kit Tilly
for critical review of the manuscript. This research was supported by the
Intramural Research Program of the National Institute of Allergy and Infectious
Diseases, National Institutes of Health.
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