Introduction to In Situ Forming Hydrogels for Biomedical Applications - In-Situ Gelling Polymers: For Biomedical Applications - page 26

(a)

(b)

70

PI PII PIII Matrigel

60

50

40

30

20

10

0

14d 28d

(c)

PI PII PIII M

Type II Collagen

Type I Collagen

GAPDH

Fig. 15 a Nanofibrous interior morphology of (L/DL)-PA-Pluronic ® -(L/DL)-PA thermogel.
Expression of chondrogenic markers of ( b ) sulfated glycosaminoglycan (sGAG) and ( c ) type II
collagen. Matrigel TM was used as a control for 3D chondrocytes culture. Glyceraldehyde 3-phos-
phate dehydrogenase (GAPDH) is compared as a house-keeping gene. Reproduced from [ 3 ]

terminal groups modified PNIPAAm brushes were fabricated for improving cell
adhesion and cell sheet harvest [ 126 ]. Specifically, carboxyl-terminated PNIPAAm
brush surface most enhanced cell adhesion and cell sheet harvesting. (L/DL)-
PA-Pluronic ® -(L/DL)-PA thermogel showed a higher population of nanofibrous
structures as the L-Ala content increased and chondrocytes cultured in these ther-
mogels expressed higher chondrogenic markers compared to commercially avail-
able Matrigel TM (Fig. 15 ) [ 3 ].