Exploring the Molecular Basis of Insecticide Resistance in the Dengue Vector Aedes aegypti - Recent Advances and Issues in Environmental Science - page 178

Validation of microarray data was performed by real-time quantitative RT-PCR
on 10 detoxifi cation genes identifi ed as over-transcribed in larvae or adults of the
Vauclin strain (Figure 3). The over-transcription of genes identifi ed from microarray
experiments were all confi rmed by quantitative RT-PCR in both life stages, although
expression ratios obtained from RT-PCR were frequently higher than those obtained
from microarray experiments.
Figure 3. Real-time quantitative RT-PCR validation of microarray data. Validation of differential
transcription between the two strains was performed on 11 selected genes in 4th-stage larvae (white
dots) and 3-days old adults (black dots). Transcription ratios obtained from real-time quantitative
RT-PCR experiments were normalized with the two housekeeping genes AeRPL8 and AeRPS7 and
shown as mean value over 3 independent biological replicates.
DISCUSSION
The aim of the present study was to investigate insecticide resistance mechanisms of
Ae. aegypti mosquitoes from Martinique (French West Indies).
Toxicological results confi rmed the high level of resistance of the Vauclin strain
from Martinique to the organophosphate temephos at the larval stage and to the