Exploring the Molecular Basis of Insecticide Resistance in the Dengue Vector Aedes aegypti - Recent Advances and Issues in Environmental Science - page 175

Comparison of constitutive detoxifi cation enzyme activities between the susceptible
strain Bora-Bora and the insecticide-resistant Vauclin strain revealed signifi cant differ-
ences at both larval and adult stages (Figure 1). The P450 activities were elevated in
both larvae and adults of the Vauclin strain (1.57-fold and 1.78-fold respectively with

Figure 1. Comparison of detoxification enzymes activities between the insecticide-resistant strain
Vauclin and the susceptible strain Bora-Bora. ( A) The P450 activities were measured with the ECOD
method [63] on 20 μg microsomal proteins after 15 min and expressed as pmol of 7-OH produced/
mg microsomal protein/min (± SE). (B) The GST activities were measured with the CDNB method
[64] on 200 μg cytosolic proteins during 1 min and expressed as nmol of conjugated CDNB/μg
protein/min (± SE). -esterase (D) activities were measured with the naphthyl

acetate method [65] on 30 μg cytosolic proteins after 15 min and expressed as μmol

α -esterase (C) and β

-naphthol
produced/mg protein/min (± SE). For each strain and each life stage, three independent biological
replicates were analyzed and measures were repeated 15, 15 and 30 times for P450, GST, and
esterase activities respectively. Statistical comparison of enzyme activities between the Vauclin and
Bora-Bora strains were performed at each life stage separately with a Mann and Whitney's test (* p
< 0.05, ** p < 0.01, *** p < 0.001).

α - or β