Biomedical Engineering Reference
In-Depth Information
Target cell/tissue
Protein extract
Separation of proteins
(2D electrophoresis or HPLC)
Sequencing studies
(Mass spectrometry or Edman
degradation)
Identification
(sequence homology searches)
Figure 4.4
The proteomics approach. Refer to text for details
the (protein) staining threshold. The latter point can be particularly signifi cant in the context of
drug/target identifi cation, as most such targets are likely to be kinases and other regulatory pro-
teins that are generally expressed within cells at very low levels.
More recently, high-resolution chromatographic techniques (particularly reverse-phase and ion
exchanged-based high-performance liquid chromatography (HPLC)) have been applied in the
separation of proteome proteins and high-resolution mass spectrometry is being employed to aid
high-throughput sequence determination.
4.6 Structuralgenomics
Related to the discipline of proteomics is that of structural genomics. The latter focuses upon the
large-scale systematic study of gene product structure. While this embraces rRNA and tRNA,
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