Role of Mechanical Stimulations in Directing Mesenchymal Stem Cell Adipogenesis - The Mechanobiology of Obesity and Related Diseases - page 71

Fig. 4 Compression of SGBS preadipocytes reduces their adipogenesis. Experimental design is
shown. a Quantified lipid accumulation data show that cell compression before the adipogenic
induction inhibits adipogenesis. b aP2 adipogenic gene shows similar reduction under
compression. c Cell staining by DAPI (blue) and Sudan III (red). Cell number positive for
lipid staining was decreased by compression. d Optical images. e Compression (black bar)
decreased mRNA levels of adipokines and lipogenic markers. *: p \ 0.05 compared with the
control; n.s. not significant. Reprinted with permission from Elsevier [ 19 ]
chondrogenic genes (a-catenin, cathepsinB, aggrecan, BMP6) by compressive
loading. On the other hand, cell stretching increased osteogenesis while decreasing
chondrogenesis. They proposed that this difference is due to altered catenin