Role of Mechanical Stimulations in Directing Mesenchymal Stem Cell Adipogenesis - The Mechanobiology of Obesity and Related Diseases - page 66

Fig. 2 Cyclic cell stretch suppresses BMP4 induction of MSC adipogenesis. a C3H10T1/2
MSCs were grown for 4 days and then adipocytic differentiation was induced for 8 days. Cyclic
stretch (10 % strain, 0.25 Hz) was applied during the BMP4 treatment stage. b BMP4-triggered
adipogenic gene expressions were significantly reduced by stretch. Quantitative RT-PCR:
comparisons with the control (*, **) and BMP4 (#, ##) shown at p \ 0.05 (*, #) and p \ 0.01
(**, ##). c BMP4 induced lipid synthesis (oil red O staining), which was suppressed by cell
stretch. Reprinted with permission from Elsevier [ 24 ]
was demonstrated that BMP4 may also play a critical role in inducing MSC
commitment toward adipocytes. An exposure of proliferating C3H10T1/2 murine
MSCs to BMP4 induced their preadipocytic commitment, and these cells under-
went terminal adipogenesis when further treated with adipogenic hormonal
inducers [ 5 ]. Accordingly, exposure of C3H10T1/2 MSCs to Noggin, a BMP4
inhibitor, blocked subsequent adipocytic differentiation [ 6 ].
Our study demonstrated that cyclic cell stretch could suppress the BMP4
induction of MSC adipogenesis. As shown in Fig. 2 , BMP4 could trigger
C3H10T1/2 MSC adipogenesis, which effect was diminished by cyclic cell stretch