Introduction - Determination of Anions in Natural and Treated Waters - page 42

standard disposable extraction columns, Analytichem Bond-Elut Baker SPE, Supelco
Supelclean, Alltech Extract Clean, etc. There is a choice of more than 20 different
stationary phases.

Spectrofluorimetric detectors
A spectrofluorimeter has been used as a detector in the high performance liquid
chromatographic separation of polyaromatic hydrocarbons in water samples. A great
improvement in sensitivity and specificity can be obtained by the correct wavelengths.

Amino acid analysers
This is an example of a dedicated application of high performance liquid
chromatography. Measurements of amino acids have been performed in lake water and
sea water. Also, measurements of total protein or amino acids are required in river waters
and effluents.
The most popular current techniques for amino acid analysis rely on liquid
chromatography and there are two basic analytical methods. The first is based on ion
exchange chromatography with post-column derivatisation. The second uses pre-column
derivatisation followed by reversed-phase high performance liquid chromatography.
Derivatisation is necessary because amino acids, with very few exceptions, do not

Table 1.8 Detectors used in high performance liquid chromatography

Type of detector Wavelength

range (nm)

Supplier Detection part no HPLC

instrument
part no

Spectrophotometric
(variable wavelength)

190-390 Perkin-

Elmer

LC-90 -

193-350 Kontron 735 LC Series 400

195-350 Shimadzu SPD-7A LC-7A

195-350 Shimadzu SPD-6A LC-8A

195-350 Shimadzu SPD-6A LC-6A

206-405 LKB 2510Uvicord SD -

(choice of seven
fixed
wavelengths)

190-370 Cecil

Instruments

Model 1937 Chrom-A-

Scope

190-400 CE 1220 Series 100

Variable wavelength
UV-visible

190-600 Varian 2550 2500

190-600 LKB 2151 Uvicord SD