Biomedical Engineering Reference
In-Depth Information
Fig. 3
Single strand conformation polymorphism (SSCP) gel of 16S rRNA gene amplicons of
biofilm communities from patients with peri-implantitis. Each lane stands for one biofilm commu-
nity, each band is one distinct bacterial species. Bands migrating to the some height are identical
species. Main bands have been cut out, their DNA sequenced and the species identified. Some of
the identified bands are shown
study no member of the bacterial group most closely related to periodontitis was
observed but in some biofilm communities
Streptococcus mutans
, known to be the
leading pathogen in the formation of dental cavities, was detected. In general was
the diversity of
Streptococcus
and
Fusobacterium
species in peri-implantitis biofilms
much higher than in asymptomatic ones. Comparing biofilm communities between
different patients led again to the impression that each patient has his own set of
dental biofilm communities. Surprising, however, was the observation that no large
differences in the biofilm communities between infected and healthy implant of the
same patient was found. The interpretation that pathogenic biofilm communities
spread over all the teeth of an individual was supported by the finding that microbial
