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Kalay et al. 2011 ; Thornton and Woods 2009 ). The finding that microcephaly
linked proteins form a common complex may explain why mutations in any of
these lead to the same disorder. The S-CAP complex also contains CP190 and
tubulin (Gopalakrishnan et al. 2011 ).
Drosophila embryos are also a rich source for centrosomes. These centrosomes
can nucleate microtubules in a cell-free system, allowing the study of the mech-
anisms of astral microtubules formation (Moritz et al. 1995 ). The function of
centrosomal complexes can be studied further by stripping the centrosome from its
PCM using high salt and then adding embryo extract and purified c-TuRC com-
plexes (Moritz et al. 1998 ). Stripping the centrosome is also useful to identify the
mechanism of recruiting other centrosomal complexes and was used to show that
Sas-4 is the S-CAP component that tethers the complex to the centrosome (Go-
palakrishnan et al. 2011 ).
Acknowledgments This material is based upon work supported by the National Science
Foundation under Grant No. MCB-1121176. Andrey Polyanovsky was supported by RFBR grant
10-04-01027
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