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Fig. 18.3 Electron
micrographs of cultured rat
sympathetic neurons in the
region of the centrosome. a A
neuron showing centrosome
consisting of two centrioles
and multiple unattached
microtubules. b A neuron
treated for 6 h with 10 lg/ml
nocodazole, rinsed free of the
drug, and permitted to
recover for 5 min.
Microtubule reassembly from
the centrosome is dramatic,
with high levels of attached
microtubules. Also apparent
are other microtubules not
directly attached to the
centrosome. These
microtubules are aligned with
the attached microtubules as
if they were once attached
and then released from the
centrosome. Analyses of
every section through each
centrosome were required to
define and score attached and
unattached microtubules.
Adapted from Yu et al.
( 1993 ). Bar, 0.4 lm
to follow the progress of a small population of microtubules nucleated at the
centrosome (Ahmad and Baas 1995 ; also see Fig. 18.4 ). After drug treatment to
depolymerize microtubules, and a few minutes of microtubule reassembly at the
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