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Fig. 1.2 Fertilization during mitosis of the zygote (a), the giant centriole (b) and a second
smaller centriolar structure (c) are observed at the two poles. Note that the two pronuclei (blue)
are not mixed and they divide separately in parallel. A low magnification image with small inset
squares outlines the approximate positions of the centriolar structures, which are shown under a
higher magnification in (b and c). Embryos were stained with rat anti-a-tubulin (red), and anti-N-
ter-Asl (purple); 4'-6-diamidino-2-phenylindole (DAPI) stains DNA
Fig. 1.3 Spermatid with a
giant centriole (GC) and a
PCL. Left, giant centriole and
PCL labeled by Ana-1-GFP
(purple). Right, cartoon
depicting the relative location
of the sperm centriolar
structures relative to the
sperm head (H) and tail (T).
(The panel on the Left is
modified from Fig. 1.2 bin
(Blachon et al. 2009 )
duplication in early embryonic development, a time when the embryonic genome
is not yet fully involved in producing the proteins necessary for development.
On the other hand, the Drosophila sperm contains two centriolar structures. The
first, termed the ''giant centriole'' (due to its exceptional length) resembles the
distal centriole found in vertebrate sperm and functions to nucleate the sperm
flagellum (Friedlander and Wahrman 1966 ; Fuller 1993 ; Krioutchkova and
Onishchenko 1999 ; Manandhar et al. 2005 ; Sun and Schatten 2007 ). A second
centriolar structure associates with the giant centriole and is termed the proximal
centriole-like (PCL) structure due to the fact that, like the vertebrate sperm
proximal centriole, it does not form a flagellum (Blachon et al. 2009 ) (Fig. 1.3 ).
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