Biology Reference
In-Depth Information
CHAPTER
6
Current NMR Strategies
for
Biomarker Discov
ery
Que N. Van
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program,
Frederick National Laboratory for Cancer Research, Frederick, MD, USA
OUTLINE
Introduction: Why NMR?
88
Targeted Metabolic Pro
ling
98
Targeted Analysis: Stable Isotope Tagging
98
NMR Hardware Advancement
88
Targeted Analysis: Metabolite Speci
c
99
Flux Analysis Using
13
C Labeling
Sample Preparation for NMR Analysis
89
99
Biological Fluids without Macromolecules
90
High-Resolution Magic Angle Spinning
(HR-MAS) NMR Spectroscopy
Biological Fluids with Macromolecules
91
101
Cells and Tissue Extracts
92
Intact Tissue for HR-MAS
93
Magnetic Resonance Spectroscopy (MRS)
104
Internal and External Chemical Shift
Standards
NMR Data Processing and Preparation
for Statistical Analysis
93
106
93
Internal Standards
Data Post-Processing
106
94
External Standard
Spectral Alignment
106
One-Dimensional NMR Methods:
1
H,
13
C,
31
P
Data Preparation for Statistical Analysis
107
94
107
Binning
1
H
94
107
Targeted/Quantitative Spectral Fitting
13
C
95
Data Normalization and Scaling
107
31
P
95
Multivariate Statistical Analysis
108
2D Methods
95
NMR Metabolite Identi
cation
108
Homonuclear 2D
96
Future Directions and Conclusion
109
96
J-Resolved Spectroscopy
COSY/TOCSY
96
References
110
Heteronuclear 2D:
1
H-
13
C HSQC
98
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