Biology Reference
In-Depth Information
blood has become an ideal specimen choice
because of the amount of information it can
potentially provide. 11 The routine use and collec-
tion of blood samples for clinical diagnostics has
provided general information on optimal meth-
odologies and potential pitfalls in collecting,
handling, and storing blood samples. Manufac-
turers of blood collection supplies, laboratory
medicine guides, and diagnostic laboratory prac-
tices and procedures are also excellent resources
for determining optimal SOPs. Table 3 provides
a brief list of websites for additional information
about establishing your SOPs.
adequate time for a clot to form. Most manufac-
turers of collection systems for serum samples
recommend 30 to 60 min at room temperature
for a clot to form and longer if the subject was
taking any kind of anticoagulant at sample
collection. Serum samples that are allowed to
sit less than 30 min are likely to retain cellular
elements and other contaminants that will have
an impact on future analysis. Samples that sit
longer than 60 min are likely to experience lysis
of cells in the clot, releasing cellular components
not usually found in serum samples. 8
Jackson and Banks 12 provide an excellent
discussion on the factors to consider when
choosing serum versus plasma. Many bio-
repositories collect both serum and plasma
from subjects to hedge against prospectively
determining the incorrect sample type needed
for an assay. Existing biospecimens can be used
Serum
Serumprovides the liquid portion of the blood
without cells and clotting factors, which must be
removed from the blood sample by allowing
TABLE 3 A Selection of Biorepositories and Other Organizations (As of August 2012) Regarding Preanalytical
Variables and Standard Operating Procedures Used in Creating Biobanks and Biorepositories
URL
Topic
http://edrn.nci.nih.gov/resources/standard-operating-
procedures
EDRN Standard Operating Procedures for Plasma and Serum
http://www.biospecimens.cancer.gov/practices/
National Cancer Institute Best Practices for biospecimens
and biobanking
http://www.stard-statement.org
Standards for the Reporting of Diagnostic Accuracy Studies
http://www.spidia.eu
Standardization and improvement of generic preanalytical
tools and procedures for in vitro diagnostics (SPIDIA)
http://www.bbmri.eu
Biobanking and Biomolecular Resources Research
Infrastructure
http://biospecimens.cancer.gov/cahub/index.htm
The Cancer Human Biobank
https://brd.nci.nih.gov/BRN/brnHome.seam
Biospecimen Research Database
http://biospecimens.cancer.gov/default.asp
List of Biorepositories complied by the National Institutes
of Health
http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/
cfcfr/cfrsearch.cfm?cfrpart
Food and Drug Administration regulations on Good
Laboratory Practice (for non-clinical labs)
ΒΌ
58
http://www.isber.org/wg/bs/
International Society for Biological and Environmental
Repositories (ISBER)
 
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