Sample Preparation in Global Metabolomics of Biological Fluids and Tissues - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 68

TABLE 1 Summary of Performance Characteristics of Sample Preparation Methods Employed for Global Metabolomics to Date

Metabolism
quenching
step

Protein
removal
ef

Metabolome
coverage

Free
concentration

In vivo
sampling

Ionization
suppression

Small volume
samples Technique Repeatability ciency

Solvent
precipitation or
extraction

Excellent but
depends on
solvent

No Limited and

only if rapid
after collection

Excellent No High Low No

Microdialysis Excellent for

polar metabolites
Poor for
hydrophobic
metabolites

Yes Yes No data Yes High High Yes e no

sample
is withdrawn

Ultra ltration Excellent for polar

metabolites
Poor for
hydrophobic
metabolites

Yes Limited and

only if rapid
after collection

Good No High High No

SPE Good Depends on

sample
pre-treatment

No Good No Medium Not evaluated

to date

No

DBS Good No No Medium No Not

evaluated
to date

Low Yes

TFC Good Not known No Medium No Medium Not evaluated

to date

Yes

SPME Medium Yes Yes Good Yes Low High Yes e no

sample
is withdrawn
for in vivo
SPME

(Table reprinted from ref. 10 , with permission from Springer Science þ Business Media. Copyright 2012.)