Biology Reference
In-Depth Information
CHAPTER
4
Sample Preparation in Global
Metabolomics of Biological
Fluids and Tissues
Dajana Vuckovic
Department of Chemistry and Biochemistry, Concordia University, Montreal, QC, Canada
OUTLINE
Abbreviations
52
Incorporating a Derivatization Step for
GC-MS Compatibility
59
Introduction
52
LiquideLiquid Extraction (LLE) Approaches
60
An Ideal Sample Preparation Method for
Global Metabolomics?
Ultra
ltration
60
52
Solid-Phase Extraction (SPE)
61
Evaporation and Reconstitution Step
61
Sample Preparation Methods for Bio
uids
55
Dilute-and-Shoot: Preferred Method for
Urine Metabolomics
Tissue Metabolomics
62
55
New Trends in Sample Preparation for
Global Metabolomics
Solvent Precipitation: Preferred Method for
Plasma, Serum, and Other Bio
64
uids
55
In Vivo Sampling: Microdialysis and Solid-
Phase Microextraction
Plasma versus Serum in Global
Metabolomics
64
56
Turbulent Flow Chromatography (TFC)
66
Choice of Anticoagulant in Global
Metabolomics
Dried Blood (or Bio
uid) Spot Analysis
66
57
Conclusions and Future Perspective
68
Protein Removal Ef
ciency and Selection
of Plasma-Precipitant Ratio
57
Acknowledgment
70
Selection of Extraction Solvent:
Metabolite Coverage and Method
Reproducibility
References
70
58
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