Biology Reference
In-Depth Information
CHAPTER
26
Mass Spectrometry e Based Approach
for Prot ein Biomarker Ver i
cation
Zhaojing Meng, Timothy D. Veenstra
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick,
Inc., Frederick National Laboratory for Cancer Research, Frederick MD, USA
OUTLINE
Abbreviations
407
Stable Isotope-Labeled Internal
Standards Used
416
Introduction
408
Bioinformatics Software for MRM-MS
Assays and Biomarker Veri
MRM-MS Assay Generation for Protein
Quantitation
cation
418
410
Selected Biomarker Veri
cation Applications
MRM-MS Assay Performance Characteristics
for Biomarker Veri
Based on MRM-MS
420
cation
411
Conclusions and Perspectives
421
Sample Enrichment Strategies for Improving
Biomarker Veri
cation
413
Acknowledgments
422
Mass Spectrometry
Based Strategies to
Improve Biomarker Veri
References
422
e
cation
414
ABBREVIATIONS
Q
quadrupole
PSA
prostate-speci
c antigen
MRM-MS
multiple-reaction monitoring
mass
CRP
C-reactive protein
e
spectrometry
MS
CV
coef
cient of variation
mass spectrometry
ELISA
LOD
limit of detection
enzyme-linked immunosorbent assays
LOQ
limit of quantitation
SRM
selected-reaction monitoring
LC
liquid chromatography
 
Search WWH ::




Custom Search