Biology Reference
In-Depth Information
CHAPTER
3
Tissue Sample Preparation
for
Proteomic Analy
sis
Xiaoying Ye
1
, DaRue A. Prieto
1
, King C. Chan
1
,
Bih-Rong Wei
2
, Josip Blonder
1
1
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program,
SAIC-Frederick, Inc., Frederick National Laboratory for Cancer Research, Frederick,
MD, USA,
2
Center for Cancer Research, National Cancer Institute,
Bethesda, MD, USA
OUTLINE
Introduction
40
Pressure Cycling Homogenizers
44
Ultrasonic Homogenizers
44
Types of Tissues Available for MS-Based
Proteomics
40
Extraction/Solubilization Buffers
44
Fresh and/or Fresh-Frozen Tissue
41
Buffers Used in Gel-Based Tissue Proteomics
45
Formalin-Fixed Paraf
n-Embedded Tissue
41
Buffers Used in Gel-Free Tissue Proteomics
45
Detergent- and Chaotrope-Based Buffers
Used in Gel-Free Tissue Proteomics
Tissue Disruption/Homogenization
42
46
Manual Tools for Tissue Homogenization
42
Aqueous/Organic SolventseBased
Buffers
42
Glass Homogenizers/Grinders
47
42
Glass-Te
on Homogenizers/Grinders
43
Concluding Remarks
48
Stainless-Steel Homogenizers/Pulverizers
Apparatuses for Tissue Homogenization
43
Acknowledgment
49
Mechanical Rotor/StatoreType
Homogenizers/Grinders
43
References
49
43
Cryogenic Homogenizers/Grinders
Bead-Beating-Based Homogenizers/
Disruptors
43
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