Biology Reference
In-Depth Information
CHAPTER
24
MicroRN
As and Biomarker
Discovery
Hans-Martin Jäck, Jürgen Wittmann
Division of Molecular Immunology, Department of Internal Medicine III, Nikolaus-Fiebiger-Center of
Molecular Medicine, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
OUTLINE
Abbreviations
379
In Situ Hybridization of miRNAs
387
Other Methods of miRNA Detection
and Quanti
cation
Introduction
380
387
Basics of miRNA Biology, Function,
and Detection
Examples for MIRNA Biomarker Discovery
Studies
380
387
What Are miRNAs?
380
miRNAs as Biomarkers for Noncancer
Diseases or Conditions
What Is Known about miRNAs in Health
and Disease?
388
382
miRNAs as Biomarkers for Precise Cancer
Classi
How Can miRNAs Be Detected
and Quanti
cation
388
ed?
382
Serum miRNAs as Noninvasive Biomarkers
389
Northern Blot Analysis of miRNAs
384
385
miRNA Microarray Analysis
Con
ict of Interest
389
Real-Time Quantitative RT-PCR
Analysis of miRNAs
Acknowledgments
389
385
385
References
389
Deep Sequencing Analysis of miRNAs
ABBREVIATIONS
RA
rheumatoid arthritis
RISC
RNA-induced silencing complex
RNAi
RNA interference
RT-PCR
reverse transcriptase polymerase chain
reaction
siRNA
small interfering RNA
3
0
UTR
3
0
untranslated region
bp
base pair
miRNA
microRNA
mRNA
messenger RNA
nt
nucleotide
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