A Role for ProteineProtein Interaction Networks in the Identification and Characterization of Potential Biomarkers - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 324

CHAPTER
21

A Role for ProteineProtein Interaction
Networks in the Identi cation and

Characteriz ation of Potential Biomarkers

Allen D. Bosley, Sudipto Das, Thorkell Andresson
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program,
SAIC-Frederick, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA

OUTLINE

Introduction 333 Yeast 2-Hybrid Method 341

Af nity Puri cation 342

Network Analysis Using ProteineProtein
Interaction 335 Addressing the Background Problem 344

ProteineProtein Interaction Databases 340 Conclusion 345

Common Experimental Methodologies to
Interrogate ProteineProtein Interactions

References 345

341

INTRODUCTION

biological process that causes the abnormality
(disease), which would lead to better predictive
power for the marker.
It can be challenging to reliably quantify every
protein in the proteome because of their wide
concentration dynamic range, post-translational
modi

The majorities of biomarkers used in diagnos-
tics today are proteins, despite the tremendous
advances in DNA, RNA, and metabolomics tech-
nologies. The advantage of using proteins as
biomarkers is that they carry out nearly all bio-
logical functions within the cell. Arguably,
a biomarker should be directly involved in the

cations that alter their properties, and
alternative splice variants that result in different
protein isoforms. Proteins are classically